Expression Profiling
Quantification of genes and transcripts across samples, groups, conditions, and experimental models.
B&B Bio Next Generation Sequencing
Transcriptome Sequencing
RNA sequencing provides a detailed view of gene activity by quantifying RNA abundance and characterizing transcript structure across research samples.
B&B Bio helps researchers, laboratories, and biotechnology companies maintain high standards of quality and reproducibility through reliable authentication workflows.
Projects are reviewed for sample type, RNA quality, library strategy, sequencing depth, comparison groups, bioinformatics scope, and required deliverables before work begins.
Service Overview
Measure Gene Activity and Characterize Expressed Transcripts
Transcriptome sequencing quantifies RNA abundance to determine relative gene expression in tumor and other research samples. It can identify gene-expression profiles, gene fusions, alternative splicing, and allele-specific expression.
RNA sequencing can also characterize immune-marker expression and support research into cellular pathways, tumor subtype classification, proliferation, immune activity, and hypotheses related to treatment response. Findings are intended for research interpretation and not for clinical decision-making unless separately validated and authorized.
Transcript Discovery
Project-specific options for fusion detection, splice-junction analysis, isoforms, and allele-specific expression.
Oncology Research
Support for tumor biology, subtype research, proliferation signatures, pathway activity, and tumor-microenvironment studies.
Defined Deliverables
Raw data, processed files, expression matrices, quality summaries, and selected reports are agreed before initiation.
RNA-Seq Capabilities
What Transcriptome Sequencing Can Reveal
Gene-Expression Profiles
Quantify RNA abundance and compare expression patterns across samples, biological groups, or experimental conditions.
- Gene- and transcript-level quantification
- Differential-expression analysis options
- Cluster and sample-similarity assessment
- Expression-signature research
Gene Fusions
Investigate known and candidate expressed fusion transcripts using project-specific analysis methods.
- Known and candidate fusion detection
- Fusion transcript review
- Project-specific filtering
- Independent confirmation planning
Alternative Splicing
Assess exon usage, splice junctions, and other transcript-structure features when included in the project plan.
- Splice-junction analysis
- Isoform assessment
- Exon-usage comparison
- Project-specific interpretation
Allele-Specific Expression
Explore allele-specific expression and imprinting-related patterns in suitable research designs.
- Allelic expression assessment
- Reference-aware analysis
- Matched data integration options
- Research interpretation
Immune-Marker Expression
Characterize immune-marker expression and transcriptional activity in tumors and research models.
- Immune-marker profiles
- Tumor-microenvironment research
- Immune-activity assessment
- Hypothesis generation
Pathway and Subtype Analysis
Support pathway research, tumor-subtype classification, proliferation studies, and functional interpretation.
- Pathway and enrichment options
- Subtype research support
- Proliferation signatures
- Functional interpretation
Transcriptome Sequencing Workflow
Project and Sample Review
Study groups, biological question, sample source, RNA type, quality, quantity, and required outputs are reviewed.
RNA QC and Library Preparation
RNA quality is assessed and an appropriate library strategy is selected, such as poly(A) enrichment or ribosomal RNA depletion.
Sequencing and Data Processing
Libraries are sequenced using the agreed configuration, followed by demultiplexing, read-level QC, and alignment or transcript quantification.
Analysis and Delivery
Expression data, selected transcript analyses, quality summaries, files, and reports are delivered according to the project plan.
Study Design
Research Study Configurations
Bulk RNA Sequencing
Transcriptome profiling of tissues, tumors, cell populations, and experimental models.
- Gene-expression quantification
- Case-control and multi-group studies
- Time-course and treatment comparisons
- Pathway and functional analysis options
Tumor Transcriptome Research
RNA profiling of tumor and matched research materials to investigate expressed molecular features.
- Fresh-frozen and FFPE project options
- Fusion and splice analysis options
- Subtype and expression-signature research
- Immune-marker and pathway assessment
Human Cell-Line and Model Research
Expression profiling of human cell lines, engineered models, clones, and treatment-response experiments.
- Parent and edited clone comparisons
- Drug-treatment and resistance studies
- Knockout and knock-in model assessment
- Integration with genome-editing services
Sample Planning
Sample Types and RNA Input Considerations
Purified Total RNA
RNA with documented concentration, volume, purity, integrity, extraction method, and storage conditions.
Fresh or Frozen Tissue
Source tissue or extracted RNA may be accepted after technical review and confirmation of preservation and quality requirements.
FFPE Tissue
FFPE-derived RNA may require specialized library preparation and project-specific quality thresholds because of RNA fragmentation.
Cells and Cell Pellets
Cultured cells, cell pellets, and other research materials may be suitable depending on extraction, yield, integrity, and study design.
Broad Expression View
Measure thousands of expressed genes and transcripts within a single sequencing workflow.
Quality Review
RNA, library, sequencing, and analysis quality are reviewed at defined stages.
Bioinformatics Options
Analysis can be tailored to expression, fusion, splicing, immune, pathway, or model-system objectives.
Confidential Handling
Research materials, project information, and data are handled according to agreed requirements.
Data Deliverables
Raw Sequencing Data
Demultiplexed FASTQ files and run-level quality information, as specified in the project plan.
Aligned or Quantified Data
BAM or CRAM files, transcript-abundance files, or gene-level count matrices aligned or quantified against the agreed reference.
Expression Analysis
Normalized expression matrices, differential-expression outputs, sample relationships, and selected visualizations when included.
Transcript Analyses
Project-specific outputs may include gene-fusion candidates, splice-junction results, isoform analysis, or allele-specific expression.
Optional Research Reports
Quality summaries, pathway or enrichment results, immune-marker summaries, and other research-focused reports may be provided by agreement.
Plan Your Transcriptome Sequencing Project
Contact B&B Bio to define sample requirements, RNA quality criteria, library strategy, sequencing depth, comparison groups, analysis scope, and deliverables.
B&B Bio Transcriptome Sequencing services are intended for research use only and are not intended for diagnostic procedures or treatment selection unless separately validated and authorized under applicable regulations. Expression signatures, immune-marker findings, pathway analyses, fusion candidates, and other outputs require appropriate research interpretation and independent confirmation where applicable. Final sample acceptance criteria, library method, sequencing configuration, analytical methods, timeline, and deliverables are established in the approved project plan.