Flexible Study Design
Germline, somatic, matched tumor-normal, human cell-line, and other research configurations.
B&B Bio Next Generation Sequencing
Whole Exome Sequencing
Focused sequencing of protein-coding regions to support genomic variant research, human cell-line studies, inherited-disease research, and oncology projects.
B&B Bio helps researchers, laboratories, and biotechnology companies maintain high standards of quality and reproducibility through reliable authentication workflows.
Every project is reviewed for sample type, DNA quality, study design, coverage objectives, sequencing configuration, bioinformatics scope, and required deliverables before work begins.
Service Overview
High-Value Genomic Information from Coding Regions
Whole Exome Sequencing enriches and sequences exons, the protein-coding portions of genes. It provides a focused approach for identifying sequence variants that may affect protein structure or function while reducing the data volume associated with whole-genome sequencing.
B&B Bio can support the complete workflow from DNA quality review and library preparation through exome capture, sequencing, quality assessment, data processing, and research-focused variant analysis.
Project-Specific Coverage
Sequencing depth and run design are established according to the biological question and sample type.
Integrated Bioinformatics
Options may include alignment, quality metrics, SNV and small-indel calling, annotation, and selected additional analyses.
Defined Deliverables
Raw and processed files, quality summaries, and project-specific reports are agreed before initiation.
Whole Exome Sequencing Workflow
Project and Sample Review
Research objectives, sample groups, reference genome, DNA source, quality, quantity, and desired outputs are reviewed.
Library Preparation and Capture
Sequencing libraries are prepared, indexed, quality checked, and enriched using an appropriate exome-capture workflow.
Sequencing and Run QC
Libraries are sequenced using the agreed configuration, followed by review of run quality, coverage, and data yield.
Analysis and Delivery
Data processing, selected variant analysis, quality summaries, files, and reports are delivered according to the project plan.
Study Design
Research Study Configurations
Germline Research
Variant discovery in constitutional DNA for inherited-disease, family, population, and model-system research.
- Individual, family, or cohort studies
- SNV and small-indel analysis options
- Annotation and filtering support
- Research-focused variant summaries
Somatic and Oncology Research
Exome profiling of tumors, matched tumor-normal pairs, and oncology research materials.
- Tumor-only or matched study designs
- FFPE and frozen tissue options
- Somatic SNV and indel analysis options
- Optional copy-number analysis by agreement
Human Cell-Line and Model Research
Genomic characterization of human cell lines, engineered models, clones, and treatment-response studies.
- Parent and edited clone comparisons
- Drug-treatment and resistance studies
- Model verification and genomic profiling
- Integration with other research services
Sample Planning
Sample Types and Input Considerations
Purified Genomic DNA
High-quality DNA with documented concentration, volume, purity, and storage conditions.
Blood or Bone Marrow
Extracted DNA or source material may be accepted after technical review and confirmation of project requirements.
Tissue, Cells, and Cell Pellets
Fresh or frozen tissues, cultured cells, and cell pellets may be suitable depending on extraction and quality needs.
FFPE-Derived DNA
Acceptance depends on DNA quantity, fragmentation, fixation history, tumor content, and project-specific performance needs.
Coding-Region Focus
Concentrates sequencing effort on exons for efficient coding-variant research.
Quality Review
Sample, library, sequencing, and analysis quality are reviewed at defined stages.
Bioinformatics Options
Analysis scope can be tailored to germline, somatic, cohort, or model-system projects.
Confidential Handling
Research materials, project information, and data are handled according to agreed requirements.
Data Deliverables
Raw Sequencing Data
Demultiplexed FASTQ files and run-level quality information, as specified in the project plan.
Aligned Data
BAM or CRAM files aligned to the agreed reference genome, when included in the analysis scope.
Variant Files
VCF files for selected SNV and small-indel workflows, with optional annotation and filtering outputs.
Quality Summaries
Project-specific metrics may include read quality, mapping performance, target coverage, duplication, and data yield.
Optional Analysis Reports
Research-focused summaries, tables, and selected additional analyses may be provided by agreement.
Plan Your Whole Exome Sequencing Project
Contact B&B Bio to define sample requirements, study design, sequencing depth, analysis scope, timeline, and deliverables.
B&B Bio Whole Exome Sequencing services are intended for research use only and are not intended for diagnostic procedures unless separately validated and authorized under applicable regulations. Final sample acceptance criteria, capture platform, sequencing configuration, analytical methods, timeline, and deliverables are established in the approved project plan.